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KMID : 0383419680090030359
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Korean Modern Medical Journal
1968 Volume.9 No. 3 p.359 ~ p.368
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Effect of Fracture and Administration of Corticosteroid on Glucose Metabolism in Mice Femur
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Abstract
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A study on the effect of fracture and administration of cortison and DOCA on glucose-C14 metabolism in the femur of mice was made through the following experiments:
One hundred and twenty male mice, weighing about 25mg each, were divided into. six groups f 20 mice respectively, and the first three groups were used for in vivo and the remaining three for in vitro experiments.
Then the three in vivo groups were subdivided for three different experimental purposes (1) Control, (2) Cortison Administration and (3) DOCA Administration experiments, and the remaining tthree in vitro groups were also subdivided for three additional purposes(4) Control, (5) Cortison ;effect and (6) DOCA effect..
In in vivo experiments, the mice of the second groupCortisonwere given subcutaneously 0.5mg of Cortison acetate injection, and those of the third group DOCA 0.lmg of DOCA injection per gram of body weight at an interval of 24 hours for three successive days.
Then, on the day of last administration of each reagent, the mice were fractured in the mid-section of their left femur, then slaughtered two days after the simple fracture. Both femora, one i.normal and the other fractured, of each slaughtered mouse were freed of soft tissue attachment, then seperately removed and pulverized, and then homogenized with an incubation medium. Then the incubation mixtures were incubated for another two hours at 380 C.
While in the experiments on the other three in vitro groups, the mice of each group were also saCtured manually in their left femur, and slaughtered two days later. Both femora
were also freed of soft tissue attachment and removed seperately, as in the case of the in vi experiment. Then the femora obtained from Control group were immersed in a soap solution a those from the group for Cortison effect in a soap solution impregnated with 15mg of Corti; acetate, and others from the group for DOCA effect in a soap solution containing 0.31ng DOCA. Then those femora were incubated for 24 hours thereafter, and also pulverized and he ogenized in an incubation medium. Then the homogenized ´mixtures were again incubated
another two hours at 3$OC.
And with the homogenates thus obtained, such facts as production rate and radioactivity CO2, relative specific activity, concentration of glucose contained in the media before and al incubation, were first measured. i
Then on the grounds of these basic measurements such things as specific activity of Q) relative specific activity, glucose consumption rate and relative glucose disappearance were calculated. and the following conclusions finally reached.
1. The Coe production rate, specific activity, relative; specific activity, glucose consumption rate" and relative glucose disappearance were increased in fracture by. administration of cortison, both in vivo and in vitro experiments, accordingly, it could be concluded that cortison directly and: remarkably elevates the glucose metabolism in bone tissue.
2. Both in viov and in. vitro, DOCA has not much effete on the oxidative metabolism of glucose in bone tissue, regardless of fractured or non-fractured condition.
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KEYWORD
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